Fig. 5.
Fig. 5. Kinetics of CD41 acquisition and CD9 modulation in megakaryocytic liquid cultures. / CD34+CD41neg cells were sorted according to CD9 expression, as shown in Figure 3, and were cultured for 6 days under megakaryocytic liquid conditions as described in “Materials and methods.” (A) Kinetics of CD9 and CD41 expression in CD34+ cell subpopulations sorted according to CD9 expression (CD9low, CD9mid, CD9high) during a 6-day culture. (B) Evolution of the percentage of CD41highCD9high cells in the different cell fractions during the culture. Plots and curves are for one experiment representative of the 4 performed using different CD34+ bone marrow samples.

Kinetics of CD41 acquisition and CD9 modulation in megakaryocytic liquid cultures.

CD34+CD41neg cells were sorted according to CD9 expression, as shown in Figure 3, and were cultured for 6 days under megakaryocytic liquid conditions as described in “Materials and methods.” (A) Kinetics of CD9 and CD41 expression in CD34+ cell subpopulations sorted according to CD9 expression (CD9low, CD9mid, CD9high) during a 6-day culture. (B) Evolution of the percentage of CD41highCD9high cells in the different cell fractions during the culture. Plots and curves are for one experiment representative of the 4 performed using different CD34+ bone marrow samples.

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