American Society of Hematology

Fig. 3.

$Fig. 3. Cell sorting gates according to CD9 expression. / Purified bone marrow CD34+ cells were gated on the FS/SS high fraction (R1 region) excluding the R2 region, corresponding to the low FS/SS pre-B cell population. CD34+ cells were further sorted according to CD9 expression and excluding CD41+cells. Gates A, B, and C corresponded to the CD9low, CD9mid, and CD9high cell populations, respectively. In some experiments, CD9+CD41+cells were sorted according to CD41 expression; gates D and C corresponded to CD41mid/low and CD41high cells, respectively. The bottom histogram shows the CD9 expression in the A-, B-, and C-sorted populations.$

Cell sorting gates according to CD9 expression.

Purified bone marrow CD34⁺ cells were gated on the FS/SS high fraction (R1 region) excluding the R2 region, corresponding to the low FS/SS pre-B cell population. CD34⁺ cells were further sorted according to CD9 expression and excluding CD41⁺cells. Gates A, B, and C corresponded to the CD9^low, CD9^mid, and CD9^high cell populations, respectively. In some experiments, CD9⁺CD41⁺cells were sorted according to CD41 expression; gates D and C corresponded to CD41^mid/low and CD41^high cells, respectively. The bottom histogram shows the CD9 expression in the A-, B-, and C-sorted populations.

This Feature Is Available To Subscribers Only