Effects of TNF-neutralizing antibodies on VEGF-induced endothelial cell permeability and proliferation.

(A) HUVECs were preincubated in the presence (closed diamonds) or absence (closed triangles) of the neutralizing TNF-specific mAb T1 (10 μg/mL) prior to treatment with VEGF (1 ng/mL) or left untreated (open diamonds), and hydraulic conductivity was determined as described in the text. (B) HUVECs were cultivated for 3 days with or without VEGF (10 ng/mL) in the presence (hatched bars) or absence (empty bars) of the neutralizing TNF-specific antibody T1 (30 μg/mL), and cells were counted. Data are presented as means (± SD) of 3 separate experiments.