Fig. 2.
Fig. 2. Autocrine activation of TNF-expressing HUVECs. / (A) TNF expression of HUVECs revealed by FACS analysis using TNF-specific mAb T1 (αTNF, solid line) versus an isotype-matched control antibody (Co, dotted line). (B) IL-6 production in the supernatants of HUVECs as determined by ELISA in the absence (empty bars) or presence (hatched bars) of the TNF-specific mAb T1 (30 μg/mL each). Shown are 5 examples of IL-6–expressing HUVECs generated from different cord donors (No. 21-36).

Autocrine activation of TNF-expressing HUVECs.

(A) TNF expression of HUVECs revealed by FACS analysis using TNF-specific mAb T1 (αTNF, solid line) versus an isotype-matched control antibody (Co, dotted line). (B) IL-6 production in the supernatants of HUVECs as determined by ELISA in the absence (empty bars) or presence (hatched bars) of the TNF-specific mAb T1 (30 μg/mL each). Shown are 5 examples of IL-6–expressing HUVECs generated from different cord donors (No. 21-36).

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