Fig. 2.
Fig. 2. Expression analysis of the candidate genes in B-CLL. / The 13q14 status of each sample is indicated: +/+, no loss;+/−, monoallelic loss; −/−, biallelic loss; nd, not determined. PolyA+ (or total, tot) RNA derived from B-CLL samples (indicated by the corresponding number) or cell lines was sequentially hybridized with A, 1B4/Leu2-; B,EST70/Leu1-; and C, CAR-specific probes (in this order). The cell lines used include 6 B-CLL-derived cell lines,353940 one B-cell lymphoma-derived line (BJAB), one cord blood-derived line (CB33), and one lymphoblastoid cell line (EREB-E238). The transcript sizes (kb) are indicated. Exposure times are 6 days for the 1B4/Leu2 gene and 2 days for the EST70/Leu1 and CAR genes. The normal retained allele of genes EST70/Leu1 and CAR is expressed in the deleted samples. On the contrary, no expression of the1B4/Leu2 gene is detected in B-CLL tumors, whereas traces of expression were detected in B-CLL-derived cell lines.

Expression analysis of the candidate genes in B-CLL.

The 13q14 status of each sample is indicated: +/+, no loss;+/−, monoallelic loss; −/−, biallelic loss; nd, not determined. PolyA+ (or total, tot) RNA derived from B-CLL samples (indicated by the corresponding number) or cell lines was sequentially hybridized with A, 1B4/Leu2-; B,EST70/Leu1-; and C, CAR-specific probes (in this order). The cell lines used include 6 B-CLL-derived cell lines,35,39,40 one B-cell lymphoma-derived line (BJAB), one cord blood-derived line (CB33), and one lymphoblastoid cell line (EREB-E238). The transcript sizes (kb) are indicated. Exposure times are 6 days for the 1B4/Leu2 gene and 2 days for the EST70/Leu1 and CAR genes. The normal retained allele of genes EST70/Leu1 and CAR is expressed in the deleted samples. On the contrary, no expression of the1B4/Leu2 gene is detected in B-CLL tumors, whereas traces of expression were detected in B-CLL-derived cell lines.

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