Fig. 10.
Proliferation of thymic emigrants in response to polyclonal stimuli.
Thymic emigrants were generated as in Figure 2. Cells were cultured (2 × 105/well) for 72 hours with (left panel) PMA, A23187, or (right panel) anti-CD3 and the indicated numbers of syngeneic dendritic cells (which were generated from bone marrow by culture in GM-CSF and IL-4). Cultures were pulsed for 6 hours with3H-TdR and incorporation was measured. For comparison, mature T cells isolated from spleen are shown.
