Effect of inhibitors of motility on thymic emigration.

Thymic lobes were cultured as in Figure 2 and beginning on day 12, various inhibitors were added (42 lobes per treatment group) and emigrants collected for the subsequent 6 hours. Treatments were control (DMSO only), cytochalasin D (2 μM), pertussis toxin (50 ng/mL), orClostridium toxin (50 ng/mL). The total number of emigrants per lobe/6 hours was control (6667), cytochalasin D (952), pertussis toxin (2619), and Clostridium toxin (2857), and, calculated from the percentage, the number of high CD3 is indicated in the lower right quadrant for each treatment group.