Fig. 7.
Emigration of positively selected CD8+ cells from Tap−/− thymic lobes following treatment with β2m.
Thymic lobes from Tap−/− mice were cultured as in Figure1. On day 12, lobes and support apparatus were transferred to a new well, human β2m was added (or omitted from the control) and emigrants collected for the subsequent 24 hours. Cells were analyzed for expression of CD8 versus TCRαβ.
