Inhibition of CCR2-dependent chemotaxis (A,B) and enzyme release (C,D).

(A) MCP-1–induced chemotaxis of monocytes in the presence (●) or absence (○) of 300 nM eotaxin. (B) Concentration-dependent inhibition of monocyte chemotaxis in response to 1 nM MCP-1 by eotaxin. (C) MCP-1 (○) or eotaxin (■) inducedN-acetyl-β-d-glucosaminidase release in cytochalasin B-treated monocytes. (D) Concentration-dependent inhibition of N-acetyl-β-d-glucosaminidase release in cytochalasin B-treated monocytes in response to 10 nM MCP-1 by eotaxin. Each panel shows mean values of 3 experiments performed with monocytes from different donors (bars indicate SEM).