¹²⁵I-MCP-1 binding competition on monocytes and CCR2-transfected cells.

Monocytes (A) or CCR2-transfected cells (B) were incubated on ice for 90 minutes with 0.8 nM ¹²⁵I-MCP-1 in the presence of increasing concentrations of unlabeled MCP-1 (○) or eotaxin (■). The cells were then pelleted by centrifugation through 6% BSA in PBS, and cell-bound radioactivity was counted. The curves (means of duplicate values) are representative of 1 of 4 experiments performed with monocytes from different donors and 3 experiments performed with CCR2 transfectants.