Fig. 4.
The inhibition of FITC–Annexin V staining by caspase inhibitors.
The apoptotic effect induced by Rituxan homodimers (peak 2) and inhibition by caspase inhibitors Z-VAD-FMK (peak 3) and Ac-YVAD-CHO (peak 4) were determined by means of the FITC–Annexin V assay. Cells at 2 × 105/mL were treated at 37°C with Rituxan homodimers (10 μg/mL) for 24 hours in the presence or absence of caspase inhibitors (50 μM), washed, and then treated with FITC–Annexin V for 15 minutes. Cells were washed again; PI was added; and the cells were then analyzed by FACScan. This is 1 representative experiment of 3.
