American Society of Hematology

Fig. 1.

$Fig. 1. HPLC purification of crude Rituxan homodimers. / Peak 1 = polymers; peak 2 = trimers; peak 3 = homodimers; peak 4 = monomers. The inset shows SDS-PAGE (4% to 15%) of the Rituxan monomers and homodimers separated by HPLC. Lane 1, Rituxan (monomer); lane 2, polymer (greater than 450 kd), HPLC fraction 1; lane 3, trimer (450 kd), HPLC fraction 2; lane 4, dimer (300 kd), HPLC fraction 3; lane 5, monomer (150 kd), HPLC fraction 4.$

HPLC purification of crude Rituxan homodimers.

Peak 1 = polymers; peak 2 = trimers; peak 3 = homodimers; peak 4 = monomers. The inset shows SDS-PAGE (4% to 15%) of the Rituxan monomers and homodimers separated by HPLC. Lane 1, Rituxan (monomer); lane 2, polymer (greater than 450 kd), HPLC fraction 1; lane 3, trimer (450 kd), HPLC fraction 2; lane 4, dimer (300 kd), HPLC fraction 3; lane 5, monomer (150 kd), HPLC fraction 4.

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