Cyclin A1 and B-myb interaction is independent of cdk2.

Baculovirus-expressed cyclin A1 was incubated with GST or GST–B-myb (aa 225-704) in the presence or absence of baculovirus-expressed cdk2. The Western blot membrane was probed with anticyclin A1 antibody. The second blot shows that cyclin A2 and B-myb did not interact even when high levels of cyclin A2 and B-myb were present. Baculovirus-expressed cyclin A2 was incubated with GST–B-myb or GST alone. The Western blot was probed with anticyclin A2 antibody.