LCH cells from eosinophilic granuloma coexpress CD1a and CD14 but not CD86 at the cell surface, and MHC class II within intracellular vesicular compartments.

(A) Fresh tissue from eosinophilic granuloma (patient 5149) was gently dissociated, and the cell suspension was washed 3 times and incubated with FITC-conjugated anti-CD1a and PE-conjugated CD14 or CD86, or with FITC and PE isotypic-matched controls. Dot plots represent the phenotype and percentages of labeled cells in the monocytic gate (R1, 29% of total events). Similar results were obtained in 2 other patients. (B) Confocal microscopy analysis of CD1a⁺ cells sorted from eosinophilic granuloma stained with FITC-conjugated anti–HLA-DR. Note the vesicular, intracytoplasmic staining.