Fig. 2.
Fig. 2. CD1a+ Langerin+ cells may express CD14 but not DC-Lamp in eosinophilic granuloma. / (A, B) Frozen sections from eosinophilic granuloma of the bone (patient 7459) were fixed in acetone and stained with anti-Langerin FITC and either CD1a-PE (A), or CD14-PE (B) and analyzed by confocal microscopy. (A): antibodies against CD1a and Langerin labeled the same cells. (B): Langerin+CD14− (arrow), Langerin+, CD14+(closed arrowhead), and Langerin− CD14+ (open arrowhead) are observed. Similar results were obtained in 3 different patients. A representative experiment is shown. Bar represents 20 μM in panel A and 10 μM in panel B. (C, D) Sections from a bone lesion (C), and a skin lesion (D) were stained with anti–DC-Lamp antibody. Although numerous positive cells are seen in the skin lesion, they are very rare in the bone lesions. Similar results were obtained in 3 different patients in each group.

CD1a+ Langerin+ cells may express CD14 but not DC-Lamp in eosinophilic granuloma.

(A, B) Frozen sections from eosinophilic granuloma of the bone (patient 7459) were fixed in acetone and stained with anti-Langerin FITC and either CD1a-PE (A), or CD14-PE (B) and analyzed by confocal microscopy. (A): antibodies against CD1a and Langerin labeled the same cells. (B): Langerin+CD14− (arrow), Langerin+, CD14+(closed arrowhead), and Langerin− CD14+ (open arrowhead) are observed. Similar results were obtained in 3 different patients. A representative experiment is shown. Bar represents 20 μM in panel A and 10 μM in panel B. (C, D) Sections from a bone lesion (C), and a skin lesion (D) were stained with anti–DC-Lamp antibody. Although numerous positive cells are seen in the skin lesion, they are very rare in the bone lesions. Similar results were obtained in 3 different patients in each group.

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