RARs are dispensable for granulopoiesis.

(A) Fluorescence-activated cell sorter (FACS) profile of WT and RARα^−/−RARγ^−/− double mutant E14.5 FL cells stained for GR-1 and Mac-1 expression. (B) RT-PCR analysis of granulocyte marker expression in E14.5 FL from WT or RARα^−/−RARγ^−/− mice. (C) May-Grünwald Giemsa staining of cells from granulocyte-macrophage colonies derived from E14.5 WT and RARα^−/−RARγ^−/− FL cells showing morphologically normal neutrophils and macrophages. (D) Absence of RARβ expression in FL or pooled colonies (C) derived from WT or RARα^−/−RARγ^−/− mutants. cDNA from E8.5 embryos (E) was used as a positive control.