Densitometric evaluation of HO-1 mRNA in HA1 cells incubated with ZnPP or ZnCl2.
HO-1 mRNA was measured by Northern analysis and normalized to the β-actin mRNA obtained from the same blot. Lane C is untreated control; lane ZP, cells incubated with 10 μM ZnPP for 24 hours; lane ZC, cells incubated with 100 μM ZnCl2 for 24 hours; lane ZP/ZC, cells incubated with 10 μM ZnPP for 6 hours then with 100 μM ZnCl2 for an additional 18 hours; lane ZC/ZP, cells incubated with 100 μM ZnCl2 for 6 hours then with 10 μM ZnPP for 18 hours. Each bar represents the mean ± SE of 3 experiments. *P < .05 versus controls; †P < .05 versus ZnPP- or ZnCl2-treated cells. Insert: Determining the maximum HO-1 mRNA induction with ZnPP incubation. Densitometric evaluation of HO-1 mRNA normalized to the β-actin mRNA. Lane C is untreated control; lane 1, cells incubated with 1 μM ZnPP for 24 hours; lane 10, cells incubated with 10 μM ZnPP for 24 hours; lane 50, cells incubated with 50 μM ZnPP for 24 hours. Maximal induction was achieved with 10 μM ZnPP.
