FcεRI-induced tyrosine phosphorylation of cellular proteins.

(A) Cells were stimulated with antigen (Ag), and total cell lysates were analyzed by anti-phosphotyrosine (top) and antiphospho-ERK immunoblotting (bottom). (B) Antigen-stimulated or nonstimulated cell lysates were immunoprecipitated (IP) with anti-PLC-γ2 antibody. Immunoprecipitates were analyzed by immunoblotting with anti-phosphotyrosine (top) and anti-PLC-γ2 antibodies (bottom). Similar results were obtained when the other cloned lines were examined in 3 independent experiments.