American Society of Hematology

Fig. 6.

$Fig. 6. Some of the myristylated Syk constitutively localizes to the GEM fraction along with Lyn, LAT, and FcεRI. / (A) The association of signaling molecules in the GEM fraction in RBL-2H3 cells. Lysates from nonstimulated RBL-2H3 cells were fractionated by sucrose density gradient centrifugation, and 12 μL each fraction was analyzed by immunoblotting with anti-pTyr, Lyn, LAT, FcεRIβ, and γ antibodies. (B) RBL-2H3 cells and cells expressingmyr-Syk (A28) were either nonstimulated or stimulated with antigen (Ag), and cell homogenates were fractionated by sucrose density gradient centrifugation and analyzed by immunoblotting with anti-Syk antibody. Similar results were obtained in 3 independent experiments.$

Some of the myristylated Syk constitutively localizes to the GEM fraction along with Lyn, LAT, and FcεRI.

(A) The association of signaling molecules in the GEM fraction in RBL-2H3 cells. Lysates from nonstimulated RBL-2H3 cells were fractionated by sucrose density gradient centrifugation, and 12 μL each fraction was analyzed by immunoblotting with anti-pTyr, Lyn, LAT, FcεRIβ, and γ antibodies. (B) RBL-2H3 cells and cells expressingmyr-Syk (A28) were either nonstimulated or stimulated with antigen (Ag), and cell homogenates were fractionated by sucrose density gradient centrifugation and analyzed by immunoblotting with anti-Syk antibody. Similar results were obtained in 3 independent experiments.

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