Schematic diagram of the mutants of Syk used in the current experiments.

Comparison of wild-type (WT) Syk and the membrane-tagged mutants. Bothmyr-Syk and myr-SykΔS are chimeric molecules of the myristylation signal from c-Src (AA 1-16) joined to the indicated portions of Syk. The myr-SykΔS lacks the 2 SH2 domains of Syk. These cDNAs were subcloned to the pSVL expression vector.