Fig. 3.
NO-induced HIF-1α accumulation is unrelated to cGMP action.
LLC-PK1 cells were exposed to 100 μM (A) or 1 mM (B) GSNO in the absence or presence of 5 μM of the guanylyl cyclase inhibitor NS 2028, preincubated for 30 minutes. HIF-1α protein was analyzed after 1, 2, or 4 hours by Western blot analysis. Blots are representative for at least 3 different experiments.
