Detection of p140/KIR3DL2 molecules on CTCL cell lines and on fresh tumor lymphocytes.

Two long-term CTCL tumor lines (Pno, Cou-L) and fresh tumor cells isolated from the blood of patient Pno (PB lymphocytes–Pno) and from tumoral skin fragments of patient Cou (skin lymphocytes) were analyzed by 2-color immunofluorescence and FACS analysis with the indicated mAbs followed by isotype-specific fluorescein isothiocyanate or phycoerythrin-conjugated goat antimouse second reagents.6,7 Contour plots were divided into quadrants representing unstained cells (lower left), cells with only red fluorescence (upper left), cells with red and green fluorescence (upper right), and cells with only green fluorescence (lower right).