Analysis of plasma fibrin monomers.

(A) Analysis of plasma run on a nonreduced 5% SDS-PAGE gel and immunoblotted with mAb Y18/HRP, directed against the N-terminus of the Aα-chain (A). (B-F) Coomassie staining of fibrin monomers run on a reduced 10% SDS-PAGE gel (B). Immunoblots of plasma, run on a reduced 10% SDS-PAGE gel, with rabbit-antihuman fibrinogen/HRP (C), mAb Y18/HRP directed against the NH₂-terminus of the Aα-chain (D), G8/HRP directed against the COOH-terminus of the Aα-chain (E). Immunoblot of fibrin monomers with rabbit-antialbumin/HRP (F). Analysis of plasma run on a nonreduced 5% SDS-PAGE gel and immunoblotted with rabbit-antihuman albumin/HRP (G). Nwg indicates fibrin(ogen)_Nieuwegein, and Co, control fibrin(ogen).