American Society of Hematology

Fig. 4.

$Fig. 4. Characterization of mutant STAT5B. / Wild-type as well as mutant STAT5B with hGM-CSFR α and βc subunits were transfected to COS7 cells. After 2 days of culture, the cells were stimulated with hGM-CSF (10 ng/mL) for 30 minutes and harvested. Cells were separated into soluble and nuclear fractions, and phosphorylation and translocation of STAT5 were analyzed by Western blotting.$

Characterization of mutant STAT5B.

Wild-type as well as mutant STAT5B with hGM-CSFR α and βc subunits were transfected to COS7 cells. After 2 days of culture, the cells were stimulated with hGM-CSF (10 ng/mL) for 30 minutes and harvested. Cells were separated into soluble and nuclear fractions, and phosphorylation and translocation of STAT5 were analyzed by Western blotting.

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