Impact of RAP on AT III effects in neutrophils.

In deactivation experiments, cells were pretreated for 20 minutes with AT III, RAP, or both concomitantly. Thereafter, IL-8–induced chemotaxis was tested (left). Chemotaxis to AT III was investigated after the treatment of cells with RAP at various concentrations (right). In addition, untreated neutrophils were allowed to migrate to various concentrations of RAP (inset). Migration time to nitrocellulose filters was 30 minutes. After fixing and staining of filters, migration depth was quantified microscopically. The chemotaxis index is the ratio between the distances of directed and undirected migration of neutrophils. Means of chemotaxis indices were compared by Mann-Whitney test after Kruskal-Wallis analysis of variance (P < .001). *P < .05; **P < .01. ns, not significant; n = 6.