Influence of IL-8 receptor blockade on AT III–induced chemotaxis.

Neutrophils were treated for 2 hours with blocking mAbs to both IL-8 receptors (CXCR1, CXCR2). An isotype-matched control mAb to IL-1 receptor served as the control. After washing, cells migrated to medium, IL-8, fMLP, or AT III for 30 minutes into nitrocellulose filters. Migration depth was measured by microscope. The chemotaxis index is the ratio between the distances of directed and undirected migration of neutrophils. Means of chemotaxis indices were compared by Mann-Whitney test after Kruskal-Wallis analysis of variance (P < .001). *P < .05; **P < .01;n = 9.