Selective enhancement of CFU-E formation by mDYRK-3 antisense oligonucleotides.

(A) Bone marrow cells or TAP-treated erythroid splenocytes were incubated (in PBS plus mSCF) with antisense (filled histogram) or sense oligonucleotides (hatched histogram) to mDYRK-3 transcripts and were transferred directly to colony-forming assays. Indexed in parentheses are fold-increases in CFU-E numbers that are due to exposure to antisense (vs sense) oligonucleotides. Also illustrated is the absence of effects of mDYRK-3 antisense (or sense) oligonucleotides on CFU-GM formation. (B) The results of additional independent experiments in which erythroid sphenocytes from TAP-treated mice were used and in which the additional controls of a scrambled antisense oligonucleotide, or no oligonucleotide, were included.