Analysis of the p53 and MDM2 protein levels after p53 AS electropermeabilization.

BL2, Priess, and Ramos cells were electroporated with either p53 AS, a control scrambled (SC) oligodeoxynucleotide, or medium and cultured for 4 hours before being analyzed by Western blot using IF2 anti-MDM2 mAb and DO1 anti-p53 mAb. The blots were subsequently hybridized with AC-74 anti-β-actin mAb to verify loading. The level of protein expression indicated below each band was obtained by densitometry following normalization for β-actin expression.