Analysis of MDM2 and p53 protein levels after mdm2 AS electropermeabilization.

BL2, Priess, Ramos, and Saos-2 cells were electroporated with either mdm2 AS, a control scrambled (SC) oligonudeoxycleotide, or medium and cultured for 4 hours before being analyzed by Western blot using IF2 anti-MDM2 mAb or DO1 anti-p53 mAb. The blots were subsequently hybridized with AC-74 anti-β-actin mAb to verify loading. The level of protein expression indicated below each band was obtained by densitometry following normalization for β-actin expression.