Fig. 1.
Fig. 1. Expression of MDM2 and p53 protein in BL2, Saos-2, Priess, and Ramos cell lines. / Equal quantities of protein extracts obtained from the indicated cell lines were separated on 7.5% SDS-PAGE, transferred to PVDF membranes, and probed with either an anti-MDM2 mAb (IF2) or anti-p53 mAb (DO1). After incubation with a rabbit antimouse IgG, the blots were revealed with [125I]-protein A and submitted to autoradiography.

Expression of MDM2 and p53 protein in BL2, Saos-2, Priess, and Ramos cell lines.

Equal quantities of protein extracts obtained from the indicated cell lines were separated on 7.5% SDS-PAGE, transferred to PVDF membranes, and probed with either an anti-MDM2 mAb (IF2) or anti-p53 mAb (DO1). After incubation with a rabbit antimouse IgG, the blots were revealed with [125I]-protein A and submitted to autoradiography.

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