Fig. 2.
Fig. 2. Effect of Egr-1 on the proliferation of BM progenitor cells of myeloid- enriched BM in secondary clonogenic assays. / (A) Eight-day-old G418-resistant BMneo and BMEgr-1 colonies formed in the primary methylcellulose cultures supplemented with IL-3 (10% WEHI-3B–conditioned medium) were each pooled, 1000 cells/dish were seeded in methylcellulose supplemented with 10% IL-3 and G418 (650 μg/mL), and incubation was allowed to proceed for another 8 days before colony scoring. Values are mean (± SD) results from 3 independent experiments. Phenotypes of cells in methylcellulose secondary colonies were differentiated. In BMneo colonies, 52% of cells were macrophages, 39% granulocytes, and 9% other cell types. In BMEgr-1 colonies, 100% of cells were macrophages. Cell phenotype was determined by cytologic analysis of May-Grünwald–stained cytospin smears prepared with cells obtained from pooled methylcellulose colonies.

Effect of Egr-1 on the proliferation of BM progenitor cells of myeloid- enriched BM in secondary clonogenic assays.

(A) Eight-day-old G418-resistant BMneo and BMEgr-1 colonies formed in the primary methylcellulose cultures supplemented with IL-3 (10% WEHI-3B–conditioned medium) were each pooled, 1000 cells/dish were seeded in methylcellulose supplemented with 10% IL-3 and G418 (650 μg/mL), and incubation was allowed to proceed for another 8 days before colony scoring. Values are mean (± SD) results from 3 independent experiments. Phenotypes of cells in methylcellulose secondary colonies were differentiated. In BMneo colonies, 52% of cells were macrophages, 39% granulocytes, and 9% other cell types. In BMEgr-1 colonies, 100% of cells were macrophages. Cell phenotype was determined by cytologic analysis of May-Grünwald–stained cytospin smears prepared with cells obtained from pooled methylcellulose colonies.

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