Fig. 1.
Effect of ectopic expression of Egr-1 on colony-forming ability of myeloid-enriched BM cell cultures.
After infection of BM cells with MSCVneo (BMneo) or MSCVneo Egr-1 (BMEgr-1), cells were assayed in methylcellulose supplemented with IL-3 (10% WEHI-3B–conditioned medium), SCM (10%), G-CSF (100 ng/mL), GM-CSF (100 ng/mL), or M-CSF (10% L-cell–conditioned medium as a source of M-CSF). Cells were seeded at concentrations of 0.5 × 105 cells/mL in 35-mm tissue-culture dishes in the presence of G418 (650 μg/mL), and colonies were scored after 8 days. Values are mean (± SD) results from 3 independent experiments.
