The WT, but not the D675G, 2NPXF, T1, or T3, SHIP construct reverts the SF-induced intracellular calcium concentration to that seen in SHIP^+/+ BMMCs.

SHIP^+/+ ( · · · · · · ) and SHIP^−/− (–-) BMMCs expressing empty vector as well as SHIP^−/− BMMCs expressing the indicated constructs (—) were preloaded with 2 μM fura-2/am and then stimulated with 400 ng/mL SF. Cytosolic calcium was measured by monitoring fluorescence intensity at 510 nm, after excitation of the sample with 2 different wavelengths (340 and 380 nm).