The T1 and T3 SHIP constructs do not revert the SF-induced PIP₃ levels to that seen in SHIP^+/+BMMCs.

(A) A schematic diagram of the second set of SHIP constructs used in this study. The SHIP lengths (in amino acids) are indicated in brackets to the right of each construct, the triangles refer to the proline-rich sequences in the C-terminus and the Ys indicate the position of the 2 NPXY motifs. (B) Western blot analysis using anti-SHIP antibodies of total cell lysates from NP40 lysed SHIP^+/+ and SHIP^−/− BMMCs expressing empty vector as well as SHIP^−/− BMMCs expressing the indicated constructs, following 2 months in suspension culture. (C) PIP₃determinations of SHIP^+/+ and SHIP^−/− BMMCs expressing empty vector as well as SHIP^−/− BMMCs expressing the T1 and T3 SHIP constructs following a 2-minute stimulation with 400 ng/mL SF. Each point is the mean ± SE of 2 determinations.