T2-SHIP in SHIP^−/− BMMCs does not become tyrosine phosphorylated in response to SF.

(A) SHIP^−/− BMMCs expressing the indicated SHIP constructs were stimulated for the indicated times (in minutes) with 400 ng/mL SF and the NP40 cell lysates subjected to immunoprecipitation with anti-SHIP antibodies and Western analysis with 4G10. (B) A reprobe of this blot with anti-SHIP antibodies demonstrates equal loading. (C) A reprobe of the same blot with anti-GFP antibodies.