Agglutination and aggregation response from platelets treated with alboaggregin A.

Washed formaldehyde-fixed platelets (700 μL) were stirred at 1100 rpm at 37°C and agglutination was induced by (1) 1 μg/mL alboaggregin A (buffer as control). Unfixed, washed platelets (700 μL) were stirred at 1100 rpm at 37°C in the presence (lower tracing) or absence (upper tracing) of 15 μg/mL echicetin (2 and 3). After 1 minute, 0.5 μg/mL alboaggregin A or 0.05 U/mL thrombin (control; 3) was added. In the same way, unfixed platelets were stirred in the presence (lower tracing) or absence (upper tracing) of Fab fragments of anti-GPVI Ab (4 and 5). After 1 minute, 0.5 μg/mL alboaggregin A or 0.36 μg/mL collagen (control; 5) was added.