Effects of anti-GPIb Abs, echicetin, and convulxin on alboaggregin A binding to fixed platelets.

Formaldehyde-fixed platelets were preincubated with increasing amounts of mAb SZ2, anti-GPIb Ab, echicetin, or convulxin and incubated with 0.5 μg/mL biotinylated alboaggregin A and streptavidin-PE. ● Platelets in the presence of echicetin, ○ platelets in the presence of convulxin, ▪ platelets in the presence of mAb SZ2, and ▴ platelets in the presence of polyclonal rabbit anti-GPIb Abs. The fluorescence of 5000 platelets per measuring point was determined by flow cytometry. Data are the mean results from 3 experiments using platelets from different donors.