SU5416 and SU6668 inhibit SCF-induced ERK1/2 phosphorylation in MO7E cells.

Serum-starved MO7E cells were treated with the indicated concentrations of SU5416 (A) or SU-A (C) for 2 hours, or SU6668 for 48 hours (B), prior to stimulation with 250 ng/mL SCF for 15 minutes. Cells were harvested and lysed. After SDS-PAGE and transfer to nitrocellulose, the blots were probed with an anti-phosphoERK1/2 antibody (top) and subsequently stripped and reprobed with an anti-ERK1/2 (bottom) antibody. The blot in panel B was subsequently reprobed with an antiactin antibody.