In vitro transcription/translation of VIIWT and VIIMT.

In vitro transcription/translation in a rabbit reticulocyte lysate system was completed with either no added DNA template (lanes 1 and 6) or with closed circular pT7VII plasmid (WT, lane 2; MT, lane 3), or with linearized pT7VII plasmid (WT, lane 5; MT, lane 4). Samples were analyzed on a 12% SDS-PAGE under reducing conditions. The difference in electrophoretic mobility is shown by arrows.