Fig. 2.
In vitro evaluation of rAAV-hFIX constructs.
Human HepG2 hepatoma cells (striped bars) and C56BL/6-derived primary myoblasts (solid bars) were seeded in 12-well plates at a density of 5 × 104 cells/well. Twenty-four hours later, the cells were exposed overnight to 1 × 1010 genomes of vectors encoding the hFIX gene under the control of either constitutive or liver-specific promoters. All transductions were performed in triplicate in a total volume of 500 μL. Twenty-four hours conditioned media were collected on day 4 after infection and assayed for hFIX by ELISA.
