Synergistic effects of IL-5 and IL-9 in eos maturation.

Cells harvested from bone marrow of naı̈ve animals (A) and peritoneal lavage of thioglycolate-injected mice (B) were cultured in medium alone (open bars), with IL-5 (hatched bars), with IL-9 (gray bars), and with the combination of IL-5 and IL-9 (black bars) for 96 hours. After 96 hours cell cultures were analyzed for total and eos numbers. *P < .05; **P < .01 denotes significant differences between the marked group and the medium controls (open bars). Values are expressed as mean ± SEM (n = 4 separate experiments). In each experiment 2 to 4 samples were pooled from each group before culture.