American Society of Hematology

Fig. 1.

$Fig. 1. Flow-FISH analysis of PBLs from a 14-year-old patient with sAANR 24 months after diagnosis. / (A) The cells were gated on region 1 (diploid cells, R1) on the basis of propidium iodide (PI) fluorescence and forward light scatter (FSC). (B) Additional regions 2 (lymphocyte gate, R2) and 3 (granulocyte gate, R3) were selected within R1 from FSC versus side scatter dot plot histograms. (C, D) Telomere fluorescence in the lymphocyte (panel C) and granulocyte (panel D) subfraction of total PBL was analyzed following hybridization with or without FITC–(C3TA2)3 PNA (dark gray and light gray histograms in panels C and D, respectively). At the time of analysis, the patients' blood counts were as follows: Hb, 8 g/dL; platelets, 19 000/μL; neutrophils, 800/μL; the difference from the age-adjusted normal telomere length for granulocytes (deltaTELgran) was − 6700 soluble fluorochrome (MESF).$

Flow-FISH analysis of PBLs from a 14-year-old patient with sAANR 24 months after diagnosis.

(A) The cells were gated on region 1 (diploid cells, R1) on the basis of propidium iodide (PI) fluorescence and forward light scatter (FSC). (B) Additional regions 2 (lymphocyte gate, R2) and 3 (granulocyte gate, R3) were selected within R1 from FSC versus side scatter dot plot histograms. (C, D) Telomere fluorescence in the lymphocyte (panel C) and granulocyte (panel D) subfraction of total PBL was analyzed following hybridization with or without FITC–(C₃TA₂)₃ PNA (dark gray and light gray histograms in panels C and D, respectively). At the time of analysis, the patients' blood counts were as follows: Hb, 8 g/dL; platelets, 19 000/μL; neutrophils, 800/μL; the difference from the age-adjusted normal telomere length for granulocytes (deltaTEL_gran) was − 6700 soluble fluorochrome (MESF).

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