Dose-response curves for CMV and control antigen in cocultures, measured with proliferation assays.

Tritium-thymidine–incorporation assays done on day 6 of coculture showed an optimal CMV-antigen concentration of 1 mg/mL with DCs as APCs (⧫). (A) Autologous monocytes pulsed with CMV antigen stimulated less proliferation when used in the same manner as APCs (●). (B) Control antigen produced minimal stimulation of proliferation with DCs as APCs (▴).