SCH66336 caused significant reductions in the viability of BCR/ABL-BaF3 cells grown in low serum and after irradiation.

Viability of BCR/ABL-BaF3 cells was assessed by trypan blue exclusion after 48 hours of growth under the conditions specified. Black bars represent cells that were not exposed to gamma irradiation; white bars indicate those that were irradiated. Viability of BCR/ABL-BaF3 cells grown in 0.1% serum plus 500 nM SCH66336 was significantly reduced from that of BCR/ABL-BaF3 cells grown in 0.1% serum without SCH66336. After irradiation, SCH66336 treatment reduced viability in BCR/ABL-BaF3 cells grown in both 10% and 0.1% serum.