IL-3 and GM-CSF are not required for induction of CML-like disease in C57Bl/6 mice by
BCR/ABL. (A) Kaplan-Meier–style survival curve for recipients of marrow transduced with p210 BCR/ABL. The survival of Balb/c WT mice is shown by the solid line. For C57Bl/6 mice (B6, dashed lines), the individual mice in each arm are indicated by symbols (squares, wild-type; circles, double-knockout), with the disease indicated by the shading. Animals that developed more than one leukemia were identified by the characteristic clinicopathologic features of each disease, as previously described.6,10 The cause of morbidity or death in mice with CML-like disease from both strains was extensive pulmonary myeloid cell infiltration (data not shown). The difference in survival between wild-type Balb/c and C57Bl/6 transplants was highly significant (P < .0001, Mantel-Cox test), whereas there was no significant difference in survival between wild-type and double-knockout C57Bl/6 transplants (P = .22). (B) Comparison of median survival (days), mean peripheral blood leukocyte count (× 103/μL), and mean spleen weight (grams, measured at the time of morbidity or death) between wild-type Balb/c (in white) and C57Bl/6 (in black) recipients of syngeneic BCR/ABL-transduced marrow. Bars indicate SE.
