Effects of pre-exposure to irrelevant adenovirus on serum neutralizing anti-adenovirus activity and the subsequent humoral anti-Id response to Id-adenovirus vaccination.
Groups of 10 mice were injected with Adβgal or PBS. Sera were collected 3 weeks later, and both groups were then vaccinated with 108 PFU Ad-IdhuCR. Nineteen days later, mice were bled again before undergoing tumor challenge 2 days later. (A) Neutralizing activity of sera from Adβgal or PBS-treated mice. Pooled sera from each group (n = 10) were serially-diluted and incubated with 50 PFU Ad-BCL1huCR. Adenovirus-serum mixture or PBS was then added to 293 cell monolayers, agar-overlayed, and incubated at 37°C until viral plaques were enumerated 6 and 8 days later. (B) Humoral anti-Id response after vaccination with Ad-IdhuCR in mice pretreated with Adβgal or PBS. Sera collected 2 days prior to tumor challenge were analyzed for anti-38C13 Id IgG antibodies by ELISA. Bars represent mean determinations in 10 animals, with error bars showing the mean ± SD.
