Immune potential of Fas-engineered T lymphocytes.

A schematic illustration of the mixed lymphocyte reactions carried out is shown (see also “Materials and methods”). Untransduced or LV′VFas-transduced primary human T cells were incubated with allogeneic PBMC for 10 days (1° MLR). Cultures were divided in half, and a second MLR was performed by another addition of allo-PBMC and in the absence or presence of 10 nM AP1903 (2° MLR). The cytotoxic activity of equivalent numbers of cells from the 1° MLR or the 2° MLR was measured in a standard chromium release assay. (A) Cytotoxic T-lymphocyte (CTL) assay with cells from the 1° MLR: specific antiallo (▴) and antiautologous (▵) response of LV′VFas-engineered cells; and specific antiallo (▪) and antiautologous (■) response of untransduced cells. (B) CTL assay with cells from the 2° MLR: specific antiallo response of LV′VFas-engineered cells cultured in the absence (▪) or the presence (▴) of 10 nM AP1903; and antiautologous control response of LV′VFas-engineered (▵) and untransduced cells (■).