AP1903-induced killing is independent of the proliferation state of human T lymphocytes.

(A) LV′VFas-transduced primary human T cells were stained for CD25 at different times after the initial stimulation, and the killing efficiency of CD25^hi (top panel) and CD25⁻(bottom panel) cells was measured 24 hours after treatment with 10 nM AP1903. Representative CD25 staining profiles (filled histograms) with the isotype control (open histograms) are shown on the left side. Survival percentages of AP1903-treated cells are shown on the right side. (B) Five, 12, or 21 days after the initial stimulation LV′VFas-transduced primary human T cells were incubated with [³H]thymidine in the absence of AP1903 to assess proliferation activity (open bars) or in the presence of 10 nM AP1903 to assess relative survival (closed bars). Values are the mean ± SD of 3 independent points.