Expression of MPL chimeric gene in mice unresponsive to PLP p139-151.

RT-PCR was performed on total RNA extracted from the spleens of mice reconstituted with MPL-PURO–infected B cells (lanes 3 to 8) and untreated control mice (lanes 1 and 2). The amplification of the housekeeping gene GAPDH was performed in each sample to ensure that equal levels of cDNA were used. Mice shown in lanes 6 to 8 were primed with PLP p139-151 and mice shown in lanes 3 to 5 were primed with KLH.