IL-2 production in response to the stimulations of PLP peptide and KLH in control and MPL-PURO–transferred mice.

IL-2 production in untreated control mice and MPL-PURO–treated mice were measured using the same preparation of LN cells used in the T-cell proliferation assay (“Materials and methods,” Figure 6). Supernatants were harvested 24 hours after initiation of cultures and IL-2 levels were determined by ELISA. IL-2 production in response to PLP p139-151 is shown in (A) and to KLH in (B). Each bar represents one mouse; the black bars represent the untreated control mice, and the gray bars represent MPL-PURO–treated mice. The concentrations of specific antigen used for stimulation are indicated in the x-axis.